Coding

Part:BBa_K4984000:Design

Designed by: Rebecca Abirached   Group: iGEM23_UConn   (2023-09-27)


Bacillus licheniformis Laccase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was initially cloned into the pET22b(+) vector using restriction sites of EcoRI and XbaI, also making this part RFC 10 compatible. The part utilized was codon optimized using the IDT Codon Optimization Tool for expression E. coli cells. The pET22b(+) vector contains ampicillin resistance and a LacI segment for selection and screening.


Source

The initial laccase gene for B.licheniformis was retrieved from the NCBI database with the accession number WP_061578657.1. The final product is 513 amino acids long.


References